Equipment

Lab-Automation

Application
example graphicI-Dot One MC: This non-contact nanoliter liquid handling platform allows the very rapid combination of different liquid components such as DNA-components. It can dispend from 2 nl up to 80 µl.
example graphicepMotion 5075TMX: We operate an epMotion 5075TMX pipetting robot. This machine can pipette, thermomix and incubate samples automatically and is especially useful for complex media setup, aliquoting and reformating of samples.
example graphicCyBio Selma: A semi-automated 96-tip pipette useful for the flexible handling of 96- and 384-well MTPs.
example graphicKröte: This liquid handling platform with four needles for pipetting, incubation and an integrated thermocycler is currently on leave to enjoy the company of the TU Darmstadt iGEM-team.
example graphic3D Printer (MakerGear M2): Our 3D printer helps us to develop custom labware for many different applications.

Bioprocessing

Application
example graphicParallel Bioreactors: We are operating 6 Multifors bioreactors, three of them equipped with off-gas analysis and massflow controllers. The system is applied for strain characterisation, optimization and small-scale cultivations.
example graphicPilot Bioreactor: Together with the Prof. Neubauer and Dr. Junne (TU-Berlin) we are operating a 15 Liter Techfors-S bioreactor, coupled to a plug-flow reactor, for scale-up/-down experiments.

Analytics

Application
HPLCHPLC: We have an old but robust PE 200 with an autosampler, RI-, UV- and fluorescence- detectors as well as a fraction collector.
GCGC: Shimadzu GC-2030 with autosampler and FID detector.
SH800SACell Sorter: For high-throughput screening and selection we operate a Sony SH800SA. All parts that come into contact with the cells can be easily exchanged, thus feel free to contact us if you want to use the machine. Currently a 488 nm and a 638 nm laser are available
PHERAstar FSXPlate Reader: PHERAstar FSX is used for microtiterplate reading. It can handle plates with up to 3456-well microplates. We have the following filters available (Ex nm/Em nm) [available from our friends at Fachhochschule Darmstadt]:
  • Fluorescence: 485 / 520
  • Fluorescence: 575 / 620
  • Fluorescence (FRET): 430 / 530 / 480
  • Fluorescence [FH]: 280 / 530 / 350
  • Fluorescence [FH]: 350 / 450
  • Fluorescence Polarization: 485 / 520 / 520
  • Fluorescence Polarization [FH]: 540-20 / 590-20 / 590-20
  • Fluorescence Polarization [FH]: 590-50 / 675-50 / 675-50
  • Time Resolved Fluorescence: 337 / 615
  • TR-FRET/HTRF: 337 / 665 / 620
  • AlphaLISA: 680 / 615

Standard Equipment

Everything for standard molecular biology (thermocycler, electrophoresis, electroporation, ...) and microbiology (shaking, cooling, disrupting [up to 2x 96 samples], ...).

Databases

Sha1NR (only internal)