Equipment
Lab-Automation
| Application |
| Hydrocycler 4: The Hydrocycler is an automated water bath which allows scaling of thermocycling to over 6ooo reactions per run by accomodating up to four 1536 well plates. |
| epMotion 5075TMX: We operate an epMotion 5075TMX pipetting robot. This machine can pipette, thermomix and incubate samples automatically and is especially useful for complex media setup, aliquoting and reformating of samples. |
| CyBio Selma: A semi-automated 96-tip pipette useful for the flexible handling of 96- and 384-well MTPs. |
| 3D Printer (MakerGear M2): Our 3D printer helps us to develop custom labware for many different applications. |
Bioprocessing
| Application |
| Parallel Bioreactors: We are operating 6 Multifors bioreactors, three of them equipped with off-gas analysis and massflow controllers. The system is applied for strain characterisation, optimization and small-scale cultivations. |
| Pilot Bioreactor: Together with the Prof. Neubauer and Dr. Junne (TU-Berlin) we are operating a 15 Liter Techfors-S bioreactor, coupled to a plug-flow reactor, for scale-up/-down experiments. |
Analytics
| Application |
| HPLC: Our Agilent 1260 Bioinert HPLC allows us to seperate both small molecules as well as proteins. Attached can be DAD-, RI-, UV- and fluorescence detectors. |
| GC: Shimadzu GC-2030 with autosampler and FID detector. |
| Cell Sorter: For high-throughput screening and selection we operate a Sony SH800SA. All parts that come into contact with the cells can be easily exchanged, thus feel free to contact us if you want to use the machine. Currently a 405 nm, 488 nm and a 638 nm laser are available. |
| Plate Reader: Extremely fast BMG Labtech PHERAstar FSX is used for microtiterplate reading. It can handle plates with up to 3456-well microplates. We have the following filters available (Ex nm/Em nm) [available from our friends at Fachhochschule Darmstadt]:
- Fluorescence: 485 / 520
- Fluorescence: 575 / 620
- Fluorescence (FRET): 430 / 530 / 480
- Fluorescence [FH]: 280 / 530 / 350
- Fluorescence [FH]: 350 / 450
- Fluorescence Polarization: 485 / 520 / 520
- Fluorescence Polarization [FH]: 540-20 / 590-20 / 590-20
- Fluorescence Polarization [FH]: 590-50 / 675-50 / 675-50
- Time Resolved Fluorescence: 337 / 615
- TR-FRET/HTRF: 337 / 665 / 620
- AlphaLISA: 680 / 615
|
| Plate Reader: BMG CLARIOstar with LVM monochromator (almost as good as filters) for microplate reading outside our robotic platform for plates up to 1536-well. |
Standard Equipment
Everything for standard molecular biology (thermocycler, electrophoresis, electroporation, ...) and microbiology (shaking, cooling, disrupting [up to 2x 96 samples], ...).